This animation summarises the key findings from a study conducted at Imperial College London investigating the challenges facing healthcare professionals who are trying to optimise antibiotic use in hospitals across different countries. The study is part of a PhD study investigating the Impact of Culture and Team Dynamics on the Implementation of Antimicrobial Stewardship Programmes. It was funded by research grants through the Economic and Social Science Research Council (ESRC) and the National Institute for Health Research (NIHR).